Pre-Clinical Testing → Example of In Vitro Study for Efficacy

An example of in vitro study for efficacy – concentration response curve

A concentration-response curve is often used to study the efficacy of a drug at different concentrations. From the MTT study, we have determined that drug candidate at 12.5 µM is tolerated by cells. We therefore assess the effect of drug on the cells at a few concentrations lower than 12.5 µM. As the candidate drug acts to increase the release of FactorX, we therefore measure the concentration of FactorX in cell culture media as an indicator of drug efficacy. The concentration-response curve would generally display a S-shape. From this set of data, we can conclude that the drug reaches its highest efficacy at 12.5 µM concentration.